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Cat No | BST-008 |
Conjugate | |
Type | 固定液 |
Source | N/A |
Size | 50mL |
Application | IHC, FCM |
Format | Liquid |
Storage | Store at 2-8℃. |
Synonyms | Fixed fluid (flow cytometry);flow cytometry Fixative solution;Fixation Buffer(flow cytometry);Fixative solution (FCM);固定液(流式细胞);流式细胞固定液; |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 实验方法: 1. 收获待测细胞, 1000 rpm离心10分钟, 弃上清. 2. 加入500ul预冷 ( 4℃) 固定液. 3. 室温孵育10分钟. 4. 1000 rpm离心10分钟. 5. 弃上清, 加入PBS洗一次, 1000rpm离心10分钟. 6. 弃上清, 加入1.5 ml破膜剂. 7. 室温孵育3-5 分钟. 8. 1000 rpm离心5 分钟. 9. 弃上清, 加入适量细胞洗液重悬细胞. 10. 加入检测抗体进行染色后, 用流式细胞仪进行分析. |
Background | Fixed fluid and membrane-breaking agent are used for cell membrane treatment before intracellular cytokine staining. |