Biotyscience Inc
Tel 400-669-8850
E-mail info@biotyscience.com
QQ 499854788
82458988
Cat No | BSB-021 |
Conjugate | |
Type | 封闭用血清 |
Source | Mouse |
Size | 5ml |
Application | IHC, IF |
Format | Liquid |
Storage | Store at -20 ℃, and avoid repeated freezing and thawing after reconstitution. |
Synonyms | 小鼠血清封闭液;正常血清;动物非免疫血清(小鼠);Normal mouse serum |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 封闭血清适用于免疫组化或者免疫荧光实验中样本的封闭.可以封闭待检样本中与蛋白质非特异性结合的位点, 另外还可以封闭样品中内源性的 Fc 片段结合位点, 阻断抗体与样品中的 Fc 受体结合, 从而降低背景, 减少假阳性.在选择封闭血清时, 要注意封闭用血清中不能含有目标蛋白, 且与一抗来源不同, 可用与二抗相同来源的封闭血清.使用方法 免疫组化: 用5-10%正常小鼠血清封闭(1: 10-1: 20倍稀释) , 室温孵育5-10分钟.清除血清, 勿洗, 滴加适当比例稀释的一抗或一抗工作液, 37℃或室温孵育4℃过夜. 细胞涂片: 用5-10%正常小鼠血清封闭(1: 10-1: 20倍稀释) , 使用 PBS 稀释( 0.01mol/L pH7.4) , 少量点滴: 漠过细胞涂片即可. |
Background | Whole serum is obtained from non-immunized (preimmune) healthy mouses. The serum is clarified through a 0.45 micron low protein binding filter. |