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WB试剂

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PMSF(100mM)

Cat NoBSQ-002
Conjugate
TypeProtein extraction and quantification
SourceN/A
Size100ml
ApplicationWB
FormatLiquid
ConcentrationPlease refer to the vial lable for the specific concentration.
StorageStore at 2-8℃ for several months and -20℃ for long time.
SynonymsPMSF
Purification>99%
NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
MolecularWeight174.19
DescriptionPMSF, 化学物质苯甲基磺酰氟的英文简称, 常用生化试剂, 用于抑制蛋白酶.
使用介绍:
1. 抑制丝氨酸蛋白酶(如胰蛋白酶, 胰凝乳蛋白酶, 凝血酶)和巯基蛋白酶(如木瓜蛋白酶).
2. 试剂配制的摩尔浓度为100mM.
3. 工作浓度1mM, 按照比例加入到WIP或RIPA组织蛋白裂解液中使用.
4. 在水液体溶液中不稳定, 30分钟就会降解一半, 必须在每一分离和纯化步骤中加入新鲜的PMSF, 样品处理超过1h, 补加一次. (见水分解, 使用前加入)
BackgroundPMSF is a inhibitor of serine proteases and acetylcholinesterase. It inhibits proteases such as chymotrypsin, trypsin, thrombin and thiol proteases such as papain, and inhibits serine proteases by sulfonating serine residues at the active site.
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