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Rat Anti-Mouse CD45R/B220 Antibody(Clone RA3.3A 1/6.1), PE/Cyanine5

Cat NoBIOS-2306F
Conjugate
Type流式抗体
CloneRA3.3A 1/6.1
SourceRat
TagPE/Cyanine5
Size1 mg
ApplicationFCM
ApplicationNoteEach lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results (Clone The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume).
FormatLiquid
Purity>90%
ConcentrationPlease refer to the vial lable for the specific concentration.
BufferSupplied in PBS; pH 7.4.
StorageStore at -20 degree for long term.
SynonymsB220
PurificationSDS-PAGE
MolecularWeight
Description
BackgroundCD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.
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