小鼠 IgG1 Fab 和 F(ab)2 制备试剂盒-其他试剂盒-北京百欧泰生物科技有限公司

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抗体产品
- 一抗
  - --|--|流式抗体
  - --|--|单克隆抗体
  - --|--|重组兔单抗
  - --|--|多克隆抗体
  - --|--|小分子抗原抗体
  - --|--|抗体对
  - --|--|标签抗体
  - --|--|修饰性抗体
  - --|--|其他抗体
- 二抗
- 试剂盒
  - --|--|标记试剂盒
  - --|--|其他试剂盒
  - --|--|标记染料
蛋白产品
生物材料
- 金属纳米材料
  - --|--|金纳米球
  - --|--|金纳米棒
  - --|--|银纳米球
  - --|--|金纳米海胆
  - --|--|碳纳米颗粒
  - --|--|特殊纳米颗粒
- 磁性微球
  - --|--|PS磁性微球
  - --|--|SiO2磁性微球
  - --|--|Fe3O4磁性微球
  - --|--|功能化磁性微球
  - --|--|Magnetic Particles Conjugates
  - --|--|琼脂糖磁性微球
  - --|--|磁性微球试剂盒
  - --|--|Fe2O3磁性微球
  - --|--|脲醛树脂UF磁性微球
  - --|--|其他磁性微球
- 单分散微球
  - --|--|聚苯乙烯微球
  - --|--|二氧化硅微球
  - --|--|PMMA微球
  - --|--|PET微球
  - --|--|PVA微球
  - --|--|PVC微球
  - --|--|PP微球
  - --|--|PE微球
  - --|--|GMA微球
  - --|--|聚乳酸PLA微球
  - --|--|PLGA微球
  - --|--|聚左旋乳酸PLLA微球
  - --|--|聚右旋乳酸PDLA微球
  - --|--|聚己内酯PCL微球
  - --|--|PTFE聚四氟乙烯微球
  - --|--|聚丁二酸丁二醇酯PBS微球
- 其他材料
  - --|--|上转换纳米颗粒
  - --|--|量子点
  - --|--|荧光微球
  - --|--|色谱填料
热销产品
- 猴痘相关产品
- 新冠相关产品
- 外泌体相关产品
- 常用试剂
  - --|--|转染试剂
  - --|--|ELISA-FCM试剂
  - --|--|WB试剂
  - --|--|IHC-ICC-IF试剂
  - --|--|载体材料

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小鼠 IgG1 Fab 和 F(ab)2 制备试剂盒

Description
Documentation
Citations

Cat No	BIOK2025
Conjugate
Type	制备试剂盒
Source	N/A
Size	10 Antibody Samples
Application	Fragmentation
Storage	Store at 2-8℃.
Synonyms	Mouse IgG1 Fab and F(ab)2 Micro Preparation Kit;小鼠 IgG1 Fab 和 F(ab) 2 微型制备试剂盒;小鼠 IgG1 Fab & F(ab) 2 制备;Fragmentation;F(ab)2 制备试剂盒;Fab 制备试剂盒;Fab2 制备试剂盒;抗体片段试剂盒
Purification
Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
MolecularWeight
Description
Background	Mouse IgG1 Fab and F(ab)2 Preparation Kit uses Immobilized Ficin to prepare fragments from mouse IgG1. Ficin generates F(ab)2 fragments exclusively in the presence of 1-4mM cysteine; and Fab fragments are generated in the presence of 25mM cysteine. Fragment generation from other IgG species and isotypes might be possible by modifying the cysteine concentration and other digestion parameters. Pepsin is commonly used for generating F(ab)2 fragments because the pepsin cleavage site on human IgG contains a Leu 234, which is conserved in most species; however mouse IgG1 lacks this residue and others, which possibly contributes to the restricted hinge region and resistance to pepsin cleaveage. Also, the low pH required for pepsin digestion can destroy or damage antibodies. For comparison, mouse IgG1 monoclonal antibodies were digested with ficin, pepsin, bromelain and elastase. Ficin digestion produced high yields of F(ab)2 fragments with the highest residual antigen-binding activity and immunoreactivity. Affinity constants of ficin-generated F(ab)2 fragments were near those of intact antibody. This kit contains the necessary components for Fab or F(ab)2 generation of mouse IgG1 and subsequent purification. Immobilized Ficin enables immediate cessation of the digestion by simply removing the resin from the antibody digest solution. The included Spin Columns allow easy manipulation of the resin and maximum Fab and F(ab)2 recoveries. The prepacked immobilized Protein A spin column and optimized binding buffer binds the intact Fc fragments and undigested IgG, allowing for efficient Fab or F(ab)2 fragments purification. The optimized cysteine concentration produces Fab or F(ab)2 with maximum purity. This complete kit makes Fab and F(ab)2 generation and purification simple, fast and effective.

BIOK2025 Mouse IgG1 Fab and F(ab)2 Preparation Preparation Kit.pdf

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小鼠 IgG1 Fab 和 F(ab)2 制备试剂盒