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Cat No | BIOK-2249 |
Conjugate | |
Type | 其他试剂盒 |
Source | N/A |
Application | Immunology; standard |
Species | 96T |
Storage | Store at 2-8 degree. |
Synonyms | 小鼠单抗Ig类/亚类/亚型鉴定用ELISA;Mouse monoclonal antibody Ig class/subclass/subtype identification ELISA kit |
Purification | |
MolecularWeight | |
Description | |
Background | This kit uses the double antibody sandwich method to identify the classes and subclasses of monoclonal antibodies or monoclonal antibodies specifically purified with affinity in the supernatant of mouse lymphocyte hybridoma culture, as well as light chain subtypes (IgG1 IgG2a IgG2b IgG3 IgM IgA Kappa Lambda can be clearly distinguished). This kit uses a secondary antibody coated microplate with a common site of mouse antibodies, which binds to mouse antibodies in the added culture supernatant. Then, HRP labeled antibodies against various types, subclasses, and subtypes of mice are added to react separately. Finally, the TMB substrate system is used to color and the absorbance of the tested monoclonal antibody is detected by an enzyme-linked immunosorbent assay to determine the class, subclass, and subtype. This reagent kit has extremely high resolution and is a reliable tool for you to identify mouse monoclonal antibody classes, subclasses, and subtypes. |