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Recombinant Human EGF Protein

Cat NoADMD-162
Conjugate
Type重组蛋白
SourceE. coli
Size1mg
ApplicationImmunology
FormatLiquid
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined bySEC-HPLC.
ConcentrationPlease refer to the vial lable for the specific concentration.
BufferSupplied in PBS
SpeciesHuman
StorageStore at -20 degree. Avoid repeated freeze/thaw cycles.
SynonymsEpidermal Growth Factor Protein, Human;HOMG4 Protein, Human;URG Protein, Human
Purification
NoteFor research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals.
MolecularWeight
Description
BackgroundEGF is the founding member of the EGF-family of proteins. Membersofthis protein family have highly similar structural andfunctionalcharacteristics. EGF contains 9 EGF-like domains and9LDL-receptorclass B repeats. Human EGF is a 6045-Da protein with53aminoacidresidues and three intramolecular disulfide bonds. As alow-molecular-weight polypeptide, EGF was first purified fromthe mousesubmandibulargland, but since then it was found in many human tissuesincludingsubmandibular gland, parotid gland. It can also be foundinhumanplatelets, macrophages, urine, saliva, milk, and plasma. EGFisagrowthfactor that stimulates the growth of various epidermal andepithelialtissues in vivo and in vitro and of some fibroblasts in cell culture. It resultsin cellular proliferation, differentiation, and survival. SalivaryEGF, whichseems also regulated by dietary inorganic iodine, also playsanimportantphysiological role in the maintenance of oro-esophageal andgastrictissue integrity. EGF acts by binding with high affinity to epidermal growthfactor receptor on the cell surface and stimulating the intrinsicproteintyrosine kinase activity of the receptor. The tyrosine kinaseactivity, inturn,initiates a signal transduction cascade that results inavarietyofbiochemical changes within the cell - a rise in intracellular calciumlevels,increased glycolysis and protein synthesis, and increasesintheexpression of certain genes including the gene for EGFR- that ultimatelylead to DNA synthesis and cell proliferation.
We can offer labeled proteins/antibodies using a broad range of intensely fluorescent dyes and labels including FITC, Biotin, Alexa Fluor, Rhodamine B, Cy, Co-Au.
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