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Cat No | BSR-004 |
Conjugate | |
Type | 抗原修复液 |
Source | N/A |
Size | 100ml |
Application | IHC-Fr, IHC-P |
Format | Liquid |
Storage | Store at 2-8 ℃. |
Synonyms | EDTA Antigen Retrieval Solution, 50× |
Purification | |
Note | 1. 本产品溶解后为高倍浓缩液, 环境温度较低时可能会有结晶析出, 可加热助溶, 待溶解完全后再行稀释使用.2. 脱蜡水化的切片, 应避免因切片干燥, 而至非特异性染色.3. 热修复操作时务必谨慎, 当心烫伤.4. 热修复时间过长易导致脱片, 请注意控制时间. |
MolecularWeight | |
Description | EDTA 抗原修复液(EDTA Antigen Retrieval solution) 是一种常用的抗原修复液, 可以用于石蜡切片、冰冻切片等样品使用多聚甲醛、甲醛或其它醛类试剂固定后的抗原修复.可以有效去除醛类固定试剂导致的蛋白之间的交联, 充分暴露石蜡切片等样品中的抗原表位, 从而大大改善免疫染色效果. |
Background | Cells or tissues fixed with paraformaldehyde, formaldehyde or other aldehyde reagents, will lead to the intracellular antigen aldehyde bond, carboxymethyl bond and closed part of the antigen determination cluster, while the crosslinking between proteins to make the antigen determination cluster hidden, resulting in weakened staining signal during immune staining, and even some false negative staining results.Therefore, it is required that when performing immunohistochemical staining, antigen repair or exposure is required first to destroy the crosslink formed between molecules during fixation, and restore the original spatial form of the antigen.Thus, we can improve the detection rate of antigen, reduce the background staining, and improve the accuracy of detection. |