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Cat No | BSB-015 |
Conjugate | |
Type | 膜再生 |
Source | N/A |
Size | 500ml |
Application | WB |
Format | Liquid |
Storage | Store at RT. |
Synonyms | Stripping buffer |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 使用膜再生液可以对同一张膜进行多次 Western Blot 检测. 在室温条件下, 将用过的膜浸泡在膜再生液 10-60 分钟, 可在不影响抗原蛋白的情况下清除膜上结合的抗体. 随后, 可以使用不同的抗体进行下一轮 Western Blot 实验. 适宜从少量样品多次检测不同蛋白质. 即使样品量并不匮乏, 采用这一策略多次检测同一 张膜上的蛋白, 能省去给药处理, 蛋白电泳和膜转移等耗费性步骤. 而且可以消除重新上样而带来的误差, 使可比性更强. |
Background | Western Blot Stripping Buffer is a gentle method for breaking antibody-antigen interactions to allow nitrocellulose and PVDF membranes to be reprobed several times using different antibodies, saving time and conserving samples. Ideal for use with chemiluminescent substrates. |