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Cat No | BIOK2023 |
Conjugate | |
Type | 制备试剂盒 |
Source | N/A |
Size | 10 Antibody Samples |
Application | Fragmentation |
Storage | Store at 2-8℃. |
Synonyms | F(ab)2 fragments Micro Preparation Kit;F(ab)2 Preparation Kit;F(ab)2 微型制备试剂盒;F(ab)2 制备试剂盒;Fragmentation;F(ab)2 制备试剂盒;Fab 制备试剂盒;Fab2 制备试剂盒;抗体片段试剂盒 |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | |
Background | F(ab)2 Preparation Kit enables efficient generation of F(ab)2 from IgG. This kit uses Immobilized Pepsin, a nonspecific endopeptidase that is active only at acid pH and irreversibly denatured at neutral or alkaline pH. Pepsin digestion typically produces a F(ab)2 fragment (~110kDa by SDS-PAGE under non-reducing conditions) and numerous small peptides of the Fc portion. The resulting F(ab)2 fragment is composed of a pair of Fab units connected by two disulfide bonds. The Fc fragment is extensively degraded and can be separated from F(ab)2 by dialysis, gel filtration or ion exchange chromatography. |