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Cat No | BIOK2016 |
Conjugate | |
Type | 其他试剂盒 |
Source | N/A |
Size | 20次 |
Application | DNA Blunting |
Format | Liquid |
Concentration | Please refer to the vial lable for the specific concentration. |
Buffer | Supplied in 335mM Tris-HCl(pH8.8 at 25℃), 33mM MgCl2, 5mM DTT, 84mM(NH4)2SO4. |
Storage | Store at -20 degree. Avoid repeated freeze/thaw cycles. |
Synonyms | DNA末端平滑试剂;DNA Blunting Kit |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 经过本试剂盒处理产生的平末端DNA片段可以用于常规的平端连接反应。粘末端有两种,一种为5 端突出末端,另外一种为3 端突出末端。本试剂盒可以将5 端突出末端补平,也可以将3 端突出末端削平(也称打平)。当末端为5 端突出末端时,T4 DNA Polymerase可以利用其5 →3 DNA聚合酶活性将末端补平;当末端为3 端突出末端时,T4 DNA Polymerase可以利用其3 →5 DNA外切酶活性将末端削平。 |
Background | DNA末端平滑试剂盒(DNA Blunting Kit)是利用T4 DNA聚合酶(T4 DNA Polymerase)的5 →3 DNA聚合酶活性和3 →5 的DNA外切酶活性,用于将酶切或PCR等反应后产生的粘末端转变为平末端的试剂盒。 |