猴胰岛素酶免试剂盒-其他试剂盒-北京百欧泰生物科技有限公司

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抗体产品
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  - --|--|流式抗体
  - --|--|单克隆抗体
  - --|--|重组兔单抗
  - --|--|多克隆抗体
  - --|--|小分子抗原抗体
  - --|--|抗体对
  - --|--|标签抗体
  - --|--|修饰性抗体
  - --|--|其他抗体
- 二抗
- 试剂盒
  - --|--|标记试剂盒
  - --|--|其他试剂盒
  - --|--|标记染料
蛋白产品
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  - --|--|金纳米棒
  - --|--|银纳米球
  - --|--|金纳米海胆
  - --|--|碳纳米颗粒
  - --|--|特殊纳米颗粒
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  - --|--|PS磁性微球
  - --|--|SiO2磁性微球
  - --|--|Fe3O4磁性微球
  - --|--|功能化磁性微球
  - --|--|Magnetic Particles Conjugates
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  - --|--|磁性微球试剂盒
  - --|--|Fe2O3磁性微球
  - --|--|脲醛树脂UF磁性微球
  - --|--|其他磁性微球
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  - --|--|GMA微球
  - --|--|聚乳酸PLA微球
  - --|--|PLGA微球
  - --|--|聚左旋乳酸PLLA微球
  - --|--|聚右旋乳酸PDLA微球
  - --|--|聚己内酯PCL微球
  - --|--|PTFE聚四氟乙烯微球
  - --|--|聚丁二酸丁二醇酯PBS微球
- 其他材料
  - --|--|上转换纳米颗粒
  - --|--|量子点
  - --|--|荧光微球
  - --|--|色谱填料
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- 常用试剂
  - --|--|转染试剂
  - --|--|ELISA-FCM试剂
  - --|--|WB试剂
  - --|--|IHC-ICC-IF试剂
  - --|--|载体材料

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猴胰岛素酶免试剂盒

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Cat No	BIOK2007
Conjugate
Type	胰岛素含量测定
Source	N/A
Size	96T
Application	ELISA
Format	Liquid
Species	Macaque
Storage	Store at 2-8°C upon receipt. Remove any unused antibodycoated strips from the microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.
Synonyms	Macaque insulin elisa kit
Purification
Note	All reagents and micro-plate strips should be equilibrated to room temperature prior to use. A standard curve must be performed with each assay. It is recommended that all standards and samples should be run in duplicate.Research Use Only. Not for Use in Diagnostic Procedures.
MolecularWeight
Description	Preparation of reagents A. 1×Wash buffer. Prepare 1×Wash buffer by mixing the 10×Wash buffer (20 ml) with 180 ml of distilled water or deionized water. The 1×Wash buffer may be stored at 2-8°C for up to one month. B. 1×Detection antibody solution. Prepare 1×Detection antibody solution by dilution of the 100×Detection antibody solution in Assay buffer, mix well. 70 μl of the 1×Detection antibody solution is required per well. Preparation of samples If a sample has a greater concentration of insulin than the highest standard, the sample should be diluted with 0 μIU/ml insulin standard solution and the assay should be repeated. Assay procedure 1. Add 70 μl of 1x Detection antibody solution to each well. 2. Add 20 μl of standard or sample to its respective well. 3.Cover the plate with a plate sealer. Incubate at room temperature for 1 hour, shaking the plate at 600 rpm on a horizontal microplate shaker. (*Alternative incubation step in the absence of shaker: gently tap the plate frame for a few seconds to ensure thorough mixing, incubate at room temperature for 2 hours.) 4. Discard well contents and remove any remaining solution by inverting and tapping the plate on a clean paper towel. Add 300 μl of 1× Wash buffer to each well. Incubate at room temperature for 30 seconds. 5. Discard the 1× Wash buffer and tap the plate on a clean paper towel to remove residual wash buffer. Repeat the wash step for a total 4 washes. 6. Add 100 μl of Substrate solution to each well, incubate at room temperature for 15 minutes. Protect from light. 7. Add 100 μl of Stop solution to each well, gently tap the plate frame for a few seconds to ensure thorough mixing. Measure absorbance of each well at 450 nm immediately. Calculation 1. Subtract the absorbance of the blank from that of standards and samples. 2. Generate a standard curve by plotting the absorbance obtained (y-axis) against insulin concentrations (x-axis) . The best fit line can be generated with any curve-fitting software by regression analysis. Log-log curvefitting is recommended for data analysis. 3. Determine insulin concentration of samples from standard curve. 4. Conversion factor: 1 μIU/ml = 0.0348 ng/ml.
Background	This assay is a sandwich ELISA based on two monoclonal antibodies against separate antigenic determinants on the insulin molecule.

BIOK2007 Macaque insulin immunoassay kit.pdf

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猴胰岛素酶免试剂盒