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Protein Marking Technology Service

 

Beijing Biotyscience Co. Ltd provides protein labeling services with a variety of labels and conjugate options, and provides customers with personalized solutions to meet their specific needs. On the basis of having a variety of options, we provide suitable labeling services. We will help you choose suitable markers or marking methods according to your specific needs and optimize the marking methods.

Description

1. Protease labeling service

The enzyme markers in the protein labeling service usually refer to horseradish peroxidase (HRP) and alkaline phospholipase (AP label). These two markers are often used to label antibodies and then used in ELISA detection. In addition, we also provide biotin labeling services for the majority of scientific researchers.

 

Type

Service Content

HRP labeling

HRP is composed of multiple isoenzymes   with a molecular weight of 40,000 and an isoelectric point of PH 3-9. The suitable   pH for enzyme catalysis varies slightly depending on the hydrogen donor, but it   is mostly around pH 5.

HRP is more suitable for rapid color reaction, the OD450 value of about   1.8-2.5 can be reached within 5-10 minutes by adding the substrate in the ELISA   test.

AP/ALP labeling

AP label is more suitable for slow color reaction, the color development   time is about 4-8 hours, the yellow compound formed by catalysis is relatively   stable, and it is suitable for quantitative detection of enzymatic kinetics.

Biotin labeling

Biotin can be used as a fusion tag to be   recombinantly expressed with the target protein for subsequent purification and   screening. For example, when screening phage libraries, it has high screening   efficiency and is also suitable for IHC tissue-specific expression monitoring.

After the protein is labeled with Biotin, it can be directly applied to   IP and magnetic bead sorting.

 

2. Protein fluorescent labeling service

Fluorescent dye-labeled protein/peptide technology is a common in vitro protein labeling technology. In addition to the fusion expression of fluorescent proteins such as GFP and GYP, the target protein can be directly labeled with fluorescent dyes for live tracking and cell sorting. For each peptide or protein that needs to be labeled, our technical staff will strictly evaluate the physical and chemical properties of the peptide or protein, such as peptide label, linker length, type and cross-linking position.

 

The fluorescent dye labeling services that Beijing Biotyscience Co. Ltd can provide include:

 

Conventional fluorescein

FITC,Rhodamine B

Cy series fluorescein

Cy3,Cy5,Cy5.5,Cy7

Alexa Fluor series fluorescein

AF350,AF488,AF555,AF594,AF647

Fluorescent protein

PE, APC

Fluorescent double label

PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7

Colloidal gold

Co-Au

 

Expression Sevice Procedures

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Sample Requirements

 

Purity> 90%

Specification>1 mg

Concentration>1 mg/ml

 

Protein: Information such as molecular weight, isoelectric point, buffer composition and pH should be provided

 

Aadvantage

Strict quality control system, skilled laboratory technicians, and rich marking experience;

Various types of markers and labeling methods are available;

Mature technical route and optimized experimental procedure to ensure a higher success rate of protein labeling;

Improve the detection sensitivity and simplify the detection workflow;

Provide one-stop technical services such as recombinant protein expression and preparation, protein sequencing, protein labeling modification, etc., saving customers valuable time and cost;

Services can be customized: prepared according to customer needs, providing customers with related services including information consultation, design and implementation of detailed experimental schemes, etc.

 

 

Order and Inquiry

 

You can place an order or Inquiry through the following methods, and we will contact you ASAP:

 

QQ 499854788

Email info@biotyscienc.com

Tel 010-5365 2239

136 8125 6816

 


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