Protein Marking Technology Service
Beijing Biotyscience Co. Ltd provides protein labeling services with a variety of labels and conjugate options, and provides customers with personalized solutions to meet their specific needs. On the basis of having a variety of options, we provide suitable labeling services. We will help you choose suitable markers or marking methods according to your specific needs and optimize the marking methods.
Description
1. Protease labeling service
The enzyme markers in the protein labeling service usually refer to horseradish peroxidase (HRP) and alkaline phospholipase (AP label). These two markers are often used to label antibodies and then used in ELISA detection. In addition, we also provide biotin labeling services for the majority of scientific researchers.
Type | Service Content |
HRP labeling | HRP is composed of multiple isoenzymes with a molecular weight of 40,000 and an isoelectric point of PH 3-9. The suitable pH for enzyme catalysis varies slightly depending on the hydrogen donor, but it is mostly around pH 5. HRP is more suitable for rapid color reaction, the OD450 value of about 1.8-2.5 can be reached within 5-10 minutes by adding the substrate in the ELISA test. |
AP/ALP labeling | AP label is more suitable for slow color reaction, the color development time is about 4-8 hours, the yellow compound formed by catalysis is relatively stable, and it is suitable for quantitative detection of enzymatic kinetics. |
Biotin labeling | Biotin can be used as a fusion tag to be recombinantly expressed with the target protein for subsequent purification and screening. For example, when screening phage libraries, it has high screening efficiency and is also suitable for IHC tissue-specific expression monitoring. After the protein is labeled with Biotin, it can be directly applied to IP and magnetic bead sorting. |
2. Protein fluorescent labeling service
Fluorescent dye-labeled protein/peptide technology is a common in vitro protein labeling technology. In addition to the fusion expression of fluorescent proteins such as GFP and GYP, the target protein can be directly labeled with fluorescent dyes for live tracking and cell sorting. For each peptide or protein that needs to be labeled, our technical staff will strictly evaluate the physical and chemical properties of the peptide or protein, such as peptide label, linker length, type and cross-linking position.
The fluorescent dye labeling services that Beijing Biotyscience Co. Ltd can provide include:
Conventional fluorescein | FITC,Rhodamine B |
Cy series fluorescein | Cy3,Cy5,Cy5.5,Cy7 |
Alexa Fluor series fluorescein | AF350,AF488,AF555,AF594,AF647 |
Fluorescent protein | PE, APC |
Fluorescent double label | PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7 |
Colloidal gold | Co-Au |
Expression Sevice Procedures
Sample Requirements
Purity> 90%
Specification>1 mg
Concentration>1 mg/ml
Protein: Information such as molecular weight, isoelectric point, buffer composition and pH should be provided
Aadvantage
Strict quality control system, skilled laboratory technicians, and rich marking experience;
Various types of markers and labeling methods are available;
Mature technical route and optimized experimental procedure to ensure a higher success rate of protein labeling;
Improve the detection sensitivity and simplify the detection workflow;
Provide one-stop technical services such as recombinant protein expression and preparation, protein sequencing, protein labeling modification, etc., saving customers valuable time and cost;
Services can be customized: prepared according to customer needs, providing customers with related services including information consultation, design and implementation of detailed experimental schemes, etc.
You can place an order or Inquiry through the following methods, and we will contact you ASAP:
QQ 499854788
Email info@biotyscienc.com
Tel 010-5365 2239
136 8125 6816
