Protein Expression Service
Protein expression system refers to a system composed of a host, foreign genes, vectors and auxiliary components. Through this system, the purpose of expressing foreign genes in the host can be achieved.
Protein expression system classification
The prokaryotic protein expression system is not only the most commonly used expression system, but also the most economical protein expression system. The prokaryotic protein expression system is represented by the E. coli expression system, which has the advantages of clear genetic background, low cost, high expression and relatively simple separation and purification of expression products.
The yeast protein expression system is represented by Pichia methanolica, which has the advantages of high expression, inducible, glycosylation mechanism similar to higher eukaryotes, easy purification of secreted proteins, and high-density fermentation.
The main advantage of the mammalian cell and insect cell expression system is that the protein post-translational processing mechanism is closest to the natural form in the body, and the biological activity is the easiest to retain.
E.coli Protein Expression Service
Service procedures | Timeline | Delivery result |
Gene synthesis and codon optimization (optional) | 1-2 weeks | Gene Synthesis Report |
Vector construction | 1-2 weeks | DH5a/TOP10 expression plasmid |
Expression strain screening | 1-2 weeks | Positive expression strain |
Protein expression and purification | 1-2 weeks | Protein production report |
Large-scale protein preparation | 1-2 weeks | Finished protein (above 85% purity) |
Beijing biotyscience also provides the following services:
Yeast protein expression system
Baculovirus-insect protein expression system
Mammalian cell protein expression system
Preparation and conjugation services of small molecule antigens
You can place an order or Inquiry through the following methods, and we will contact you ASAP:
QQ 499854788
Email info@biotyscience.com
Tel 010-5365 2239
136 8125 6816
