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Protein Expression Service

Protein expression system refers to a system composed of a host, foreign genes, vectors and auxiliary components. Through this system, the purpose of expressing foreign genes in the host can be achieved.

 

Protein expression system classification

 

The prokaryotic protein expression system is not only the most commonly used expression system, but also the most economical protein expression system. The prokaryotic protein expression system is represented by the E. coli expression system, which has the advantages of clear genetic background, low cost, high expression and relatively simple separation and purification of expression products.

The yeast protein expression system is represented by Pichia methanolica, which has the advantages of high expression, inducible, glycosylation mechanism similar to higher eukaryotes, easy purification of secreted proteins, and high-density fermentation.

The main advantage of the mammalian cell and insect cell expression system is that the protein post-translational processing mechanism is closest to the natural form in the body, and the biological activity is the easiest to retain.

 

E.coli Protein Expression Service

 

Service   procedures

Timeline

Delivery result

Gene synthesis   and codon optimization (optional)

1-2 weeks

Gene Synthesis   Report

Vector   construction

1-2 weeks

DH5a/TOP10   expression plasmid

Expression   strain screening

1-2 weeks

Positive   expression strain

Protein   expression and purification

1-2 weeks

Protein   production report

Large-scale   protein preparation

1-2 weeks

Finished   protein (above 85% purity)

 

Beijing biotyscience also provides the following services:

 

Yeast protein expression system

Baculovirus-insect protein expression system

Mammalian cell protein expression system

Preparation and conjugation services of small molecule antigens


 

Order and Inquiry

 

You can place an order or Inquiry through the following methods, and we will contact you ASAP:

 

QQ 499854788

Email info@biotyscience.com

Tel  010-5365 2239

136 8125 6816


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