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Cat No | BIOK2014 |
Conjugate | |
Type | Kit |
Source | N/A |
Size | 20次 |
Application | EMSA |
Format | Liquid |
Concentration | Please refer to the vial lable for the specific concentration. |
Buffer | Supplied in PBS. |
Storage | Store at -20 degree. Avoid repeated freeze/thaw cycles. |
Synonyms | EMSA探针生物素标记试剂盒;EMSA Probe Biotin Labeling Kit |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | Terminal Deoxynucleotidyl Transferase(TdT)可以在不依赖于模板的情况下催化在DNA的3 -OH端加上dNTP的反应。关于TdT催化双链DNA末端加dNTP的反应效率,3 端突出的双链DNA要比平端或3 端缩进的双链DNA高很多。TdT催化单链DNA末端加dNTP的反应效率要比双链DNA高很多。在适当的条件下,TdT也可以催化RNA 3 末端加NTP的反应。 |
Background | EMSA探针生物素标记试剂盒(EMSA Probe Biotin Labeling Kit)是一种通过Terminal Deoxynucleotidyl Transferase(TdT)把生物素标记的dUTP添加到单链DNA 3 末端,然后通过退火产生生物素标记的EMSA探针的试剂盒。通常DNA的3 末端被标记后不会干扰基于序列特异性蛋白结合的EMSA检测。 |